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KMID : 0616120220530030119
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Korean Journal of Pharmacognosy
2022 Volume.53 No. 3 p.119 ~ p.124
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Isolation and Synthesis of an Antifungal Metabolite Derived from the Commensal Vaginal Bacterium Dermabacter vaginalis
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So Jae-Seong
Kwon Kang-Mu
Lee Mun-Seon
Kim Dae-Keun
Hwang In-Hyun
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Abstract
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Vaginal microbiome deeply influences vaginal health via production of messenger molecules. The healthy vaginal pH ranges between 3.5 and 4.5. However, dysbiosis of vaginal microbiome increases the pH level, leading to the incidence of vaginitis. The commensal vaginal bacterium Dermabacter vaginalis―which was isolated from the vaginal fluid of a Korean female―was incubated in acidic and neutral pH to simulate healthy and vaginitis conditions, respectively. The chemical profiles of the two different cultures were compared using HPLC. The compound showing distinctive difference between the two sets of data was presumed to be a chemical messenger, which was identified as cyclo(L-pro-L-met) by analysis of NMR, MS, and specific rotation data. Synthesis was achieved in three steps (overall yield 15%), enabling structure confirmation and antimicrobial evaluation against vaginal pathogens. Cyclo(L-pro-L-met) showed antifungal activity against Candida albicans, a major cause of vulvovaginal candidiasis.
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KEYWORD
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Vaginal microbiome, Vaginal health, Dermabacter vaginalis, Cyclo(L-pro-L-met), Antifungal activity
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